Probiotics and virulent human rotavirus modulate the transplanted human gut microbiota in gnotobiotic pigs

Simple item page
dc.contributor.authorShepherd, Megan L.en
dc.date.accessioned2016-07-13T15:04:38Zen
dc.date.available2016-07-13T15:04:38Zen
dc.date.issued2014-09-09en
dc.description.abstractWe generated a neonatal pig model with human infant gut microbiota (HGM) to study the effect of a probiotic on the composition of the transplanted microbiota following rotavirus vaccination and challenge. All the HGM-transplanted pigs received two doses of an oral attenuated rotavirus vaccine. The gut microbiota of vaccinated pigs were investigated for effects of <i>Lactobacillus rhamnosus</i> GG (LGG) supplement and homotypic virulent human rotavirus (HRV) challenge. High-throughput sequencing of V4 region of 16S rRNA genes demonstrated that HGM-transplanted pigs carried microbiota similar to that of the C-section delivered baby. Firmicutes and Proteobacteria represented over 98% of total bacteria in the human donor and the recipient pigs. HRV challenge caused a phylum-level shift from Firmicutes to Proteobacteria. LGG supplement prevented the changes in microbial communities caused by HRV challenge. In particular, members of <i>Enterococcus</i> in LGG-supplemented pigs were kept at the baseline level, while they were enriched in HRV challenged pigs. Taken together, our results suggested that HGM pigs are valuable for testing the microbiota’s response to probiotic interventions for treating infantile HRV infection.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1186/s13099-014-0039-8.en
dc.identifier.urihttp://hdl.handle.net/10919/71781en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.holderZhang et al.; licensee BioMed Central Ltd.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleProbiotics and virulent human rotavirus modulate the transplanted human gut microbiota in gnotobiotic pigsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Veterinary Medicineen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/Large Animal Clinical Sciencesen

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
2014 Zhang Yuan Shepherd GP PO Probiotics and virulent human rotavirus modulate the transplanted human gut microbiota in gnotobiotic pigs.pdf
Size:
1.08 MB
Format:
Adobe Portable Document Format
Description:
Publisher's Version
Download
License bundle
Now showing 1 - 1 of 1
Name:
VTUL_Distribution_License_2016_05_09.pdf
Size:
18.09 KB
Format:
Adobe Portable Document Format
Description:
Download

Collections

All Faculty Deposits
Journal Articles, BioMed Central and SpringerOpen
Scholarly Works, Large Animal Clinical Sciences
Scholarly Works, Virginia-Maryland College of Veterinary Medicine