Expression of genes associated with apoptosis in the residual yolk sac during the peri-hatch period of broiler chicks

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dc.contributor.authorReno, Kaitlyn E.en
dc.contributor.authorCloft, Sara E.en
dc.contributor.authorWong, Eric A.en
dc.date.accessioned2023-01-09T15:56:40Zen
dc.date.available2023-01-09T15:56:40Zen
dc.date.issued2022-08-01en
dc.date.updated2023-01-09T14:46:07Zen
dc.description.abstractThe yolk sac (YS) consists of the yolk and the surrounding YS tissue, which provides essential nutrients and physiological functions for the developing embryo. After the YS is internalized into the abdominal cavity of the embryonic chick, the YS starts to degrade. Apoptosis, or programmed cell-death, is speculated to be the mechanism behind degradation of the YS. The objective of this study was to determine if degradation of the YS tissue was mediated by apoptosis during the perihatch period. The YS tissue was collected from broiler chicks from embryonic d 17 to d 7 posthatch. The mRNA abundance of genes that are involved in the regulation, initiation, and execution of apoptosis were analyzed by qPCR. The mRNA for Bcl2, Bcl2L11, cytochrome C and caspases 3, 6, 7, 8, 9, and 18 all showed a linear response from embryonic d 17 to d 7 posthatch. To confirm the role of apoptosis in the degradation of the YS tissue, a DNA fragmentation assay was performed. Degradation of genomic DNA in the YS tissue started on day of hatch. The characteristic ladder of oligonucleosomal-sized DNA fragments was observed on d 3, 5, and 7 posthatch. The combined gene expression and DNA fragmentation results demonstrate that degradation of the YS posthatch is mediated by apoptosis.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier101966 (Article number)en
dc.identifier.doihttps://doi.org/10.1016/j.psj.2022.101966en
dc.identifier.eissn1525-3171en
dc.identifier.issn0032-5791en
dc.identifier.issue8en
dc.identifier.orcidWong, Eric [0000-0002-1891-5466]en
dc.identifier.otherS0032-5791(22)00258-9 (PII)en
dc.identifier.pmid35760000en
dc.identifier.urihttp://hdl.handle.net/10919/113102en
dc.identifier.volume101en
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/35760000en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectDNA fragmentationen
dc.subjectapoptosisen
dc.subjectbroileren
dc.subjectcaspasesen
dc.subjectyolk sacen
dc.subjectGeneticsen
dc.subject1.1 Normal biological development and functioningen
dc.subject1 Underpinning researchen
dc.subjectGeneric health relevanceen
dc.subject.meshYolk Sacen
dc.subject.meshAnimalsen
dc.subject.meshChickensen
dc.subject.meshRNA, Messengeren
dc.subject.meshApoptosisen
dc.titleExpression of genes associated with apoptosis in the residual yolk sac during the peri-hatch period of broiler chicksen
dc.title.serialPoultry Scienceen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dcterms.dateAccepted2022-05-13en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/School of Animal Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen

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