Polymer characterization by size-exclusion chromatography with multi-angle light scattering (SEC-MALS): a tutorial review

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dc.contributor.authorMatson, John B.en
dc.contributor.authorSteele, Anna Q.en
dc.contributor.authorMase, Jonathan D.en
dc.contributor.authorSchulz, Michael D.en
dc.date.accessioned2024-05-02T19:37:19Zen
dc.date.available2024-05-02T19:37:19Zen
dc.date.issued2024en
dc.description.abstractThis tutorial review presents the theory and application of SEC-MALS with minimal equations and a focus on synthetic polymer characterization, serving as an entry point for polymer scientists who want to learn more about SEC-MALS. We discuss the principles of static light scattering, outline its capability to generate absolute weight-average molar mass values, and extend its application to SEC-MALS. Practical elements are emphasized, enabling researchers to appreciate how values for Mn, Mw, and Đ are determined in an SEC-MALS experiment and how experimental conditions and input values, such as the specific refractive index increment (dn/dc), influence the results. Several illustrative SEC-MALS experiments demonstrate the impact of separation quality on Mn (as opposed to Mw), the appearance of contaminants in SEC chromatograms from sample preparation, the influence of concentration on data quality, and how polymer topology affects molecular weight characterization in SEC. Finally, we address practical considerations, common issues, and persistent misconceptions.en
dc.description.versionAccepted versionen
dc.format.extentPages 127-142en
dc.format.extent16 page(s)en
dc.identifier.doihttps://doi.org/10.1039/d3py01181jen
dc.identifier.eissn1759-9962en
dc.identifier.issn1759-9954en
dc.identifier.issue3en
dc.identifier.orcidMatson, John [0000-0001-7984-5396]en
dc.identifier.orcidSchulz, Michael [0000-0001-8499-6025]en
dc.identifier.urihttps://hdl.handle.net/10919/118739en
dc.identifier.volume15en
dc.language.isoenen
dc.publisherRoyal Society Chemistryen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectGPCen
dc.subjectdispersityen
dc.subjectlight scatteringen
dc.subjectpolymer molecular weighten
dc.subjectdn/dcen
dc.titlePolymer characterization by size-exclusion chromatography with multi-angle light scattering (SEC-MALS): a tutorial reviewen
dc.title.serialPolymer Chemistryen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherJournalen
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Scienceen
pubs.organisational-group/Virginia Tech/Science/Chemistryen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Science/COS T&R Facultyen
pubs.organisational-group/Virginia Tech/Report testen

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