Optoperforation of single, intact Arabidopsis cells for uptake of extracellular dye-conjugated dextran

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dc.contributor.authorLeBlanc, Megan L.en
dc.contributor.authorMerritt, Travis R.en
dc.contributor.authorMcMillan, Jameelen
dc.contributor.authorWestwood, James H.en
dc.contributor.authorKhodaparast, Giti A.en
dc.contributor.departmentMechanical Engineeringen
dc.contributor.departmentPhysicsen
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessed2014-02-06en
dc.date.accessioned2014-03-14T15:08:14Zen
dc.date.available2014-03-14T15:08:14Zen
dc.date.issued2013-06-01en
dc.description.abstractA plant science research goal is to manipulate single cells in an intact organism in order to study their interactions with neighboring cells. Based on a technique previously demonstrated in isolated plant cells, mammalian cells and cyanobacteria, Arabidopsis epidermal cells were optoperforated to allow for uptake of external cascade blue-labeled dextrans. Adverse organelle responses were determined to be minimal and dye retention was demonstrated for at least 72 hours. This technique overcomes the physical challenges presented by the plant cell wall and demonstrates the feasibility of in situ optoperforation. (C) 2013 Optical Society of Americaen
dc.description.sponsorshipNSF IOS-0843372en
dc.description.sponsorshipU.S. Department of Agriculture 135798en
dc.description.sponsorshipInstitute of Critical Technology and Applied Sciences (ICTAS) at Virginia Techen
dc.description.sponsorshipEIGER IGERT NSF DGE-0504196en
dc.description.sponsorshipNSF-REU DMR-0846834en
dc.format.mimetypeapplication/pdfen
dc.identifier.citationMegan L. LeBlanc, Travis R. Merritt, Jameel McMillan, James H. Westwood, and Giti A. Khodaparast, "Optoperforation of single, intact Arabidopsis cells for uptake of extracellular dye-conjugated dextran," Opt. Express 21, 14662-14673 (2013); doi: 10.1364/oe.21.014662en
dc.identifier.doihttps://doi.org/10.1364/oe.21.014662en
dc.identifier.issn1094-4087en
dc.identifier.urihttp://hdl.handle.net/10919/25920en
dc.identifier.urlhttp://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-21-12-14662en
dc.language.isoenen
dc.publisherOptical Society of Americaen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectPlant-cellsen
dc.subjectLaser microbeamen
dc.subjectMicroscopyen
dc.subjectTissuesen
dc.titleOptoperforation of single, intact Arabidopsis cells for uptake of extracellular dye-conjugated dextranen
dc.title.serialOptics Expressen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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