Single-cell RNA Sequencing Reveals Heterogeneity of Cultured Bovine Satellite Cells

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dc.contributor.authorLyu, Pengchengen
dc.contributor.authorQi, Yuminen
dc.contributor.authorTu, Zhijian Jakeen
dc.contributor.authorJiang, Honglinen
dc.date.accessioned2022-01-18T02:10:12Zen
dc.date.available2022-01-18T02:10:12Zen
dc.date.issued2021-10-28en
dc.date.updated2022-01-18T02:10:08Zen
dc.description.abstractSkeletal muscle from meat-producing livestock such as cattle is a major source of food for humans. To improve skeletal muscle growth efficiency or quality in cattle, it is necessary to understand the genetic and physiological mechanisms that govern skeletal muscle composition, development, and growth. Satellite cells are the myogenic progenitor cells in postnatal skeletal muscle. In this study we analyzed the composition of bovine satellite cells with single-cell RNA sequencing (scRNA-seq). We isolated satellite cells from a 2-week-old male calf, cultured them in growth medium for a week, and performed scRNA-seq using the 10x Genomics platform. Deep sequencing of two scRNA-seq libraries constructed from cultured bovine satellite cells yielded 860 million reads. Cell calling analyses revealed that these reads were sequenced from 19,096 individual cells. Clustering analyses indicated that these reads represented 15 cell clusters that differed in gene expression profile. Based on the enriched expression of markers of satellite cells (PAX7 and PAX3), markers of myoblasts (MYOD1, MYF5), and markers of differentiated myoblasts or myocytes (MYOG), three clusters were determined to be satellite cells, two clusters myoblasts, and two clusters myocytes. Gene ontology and trajectory inference analyses indicated that cells in these myogenic clusters differed in proliferation rate and differentiation stage. Two of the remaining clusters were enriched with PDGFRA, a marker of fibro-adipogenic (FAP) cells, the progenitor cells for intramuscular fat, and are therefore considered to be FAP cells. Gene ontology analyses indicated active lipogenesis in one of these two clusters. The identity of the remaining six clusters could not be defined. Overall, the results of this study support the hypothesis that bovine satellite cells are composed of subpopulations that differ in transcriptional and myogenic state. The results of this study also support the hypothesis that intramuscular fat in cattle originates from fibro-adipogenic cells.en
dc.description.versionPublished versionen
dc.format.extent8 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifierARTN 742077 (Article number)en
dc.identifier.doihttps://doi.org/10.3389/fgene.2021.742077en
dc.identifier.eissn1664-8021en
dc.identifier.issn1664-8021en
dc.identifier.orcidJiang, Honglin [0000-0001-9540-5788]en
dc.identifier.orcidTu, Zhijian [0000-0003-4227-3819]en
dc.identifier.other742077 (PII)en
dc.identifier.pmid34777469en
dc.identifier.urihttp://hdl.handle.net/10919/107711en
dc.identifier.volume12en
dc.language.isoenen
dc.publisherFrontiersen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000717695400001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectLife Sciences & Biomedicineen
dc.subjectGenetics & Heredityen
dc.subjectFAPen
dc.subjectfibro-adipogenic progenitorsen
dc.subjectScRNA-seqen
dc.subjectcattleen
dc.subjectskeletal muscleen
dc.subjectmyoblasten
dc.subjectTRANSCRIPTOME ANALYSISen
dc.subjectMUSCLEen
dc.subjectPROGENITORSen
dc.subjectPROLIFERATIONen
dc.subjectSPECIFICATIONen
dc.subjectEXPRESSIONen
dc.subjectDISTINCTen
dc.subjectBIOLOGYen
dc.subjectFATen
dc.subject0604 Geneticsen
dc.subject1103 Clinical Sciencesen
dc.subject1801 Lawen
dc.titleSingle-cell RNA Sequencing Reveals Heterogeneity of Cultured Bovine Satellite Cellsen
dc.title.serialFrontiers in Geneticsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
dcterms.dateAccepted2021-10-11en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Animal and Poultry Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Biochemistryen
pubs.organisational-group/Virginia Tech/University Research Institutesen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciencesen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences/Durelle Scotten

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