Synthesis, cytotoxicity, and hydroxyapatite formation in 27-Tris-SBF for sol-gel based CaO-P2O5-SiO2-B2O3-ZnO bioactive glasses

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Date
2014-03-18
Journal Title
Journal ISSN
Volume Title
Publisher
Nature Publishing Group
Abstract

CaO-P2O5-SiO2-B2O3-ZnO bioactive glasses were prepared via an optimized sol-gel method. The current investigation was focused on producing novel zinc based calcium phosphoborosilicate glasses and to evaluate their mechanical, rheological, and biocompatible properties. The morphology and composition of these glasses were studied using X-ray diffraction (XRD) and scanning electron microscopy (SEM). The particle size, mechanical and flexural strength was also determined. Furthermore, the zeta potential of all the glasses were determined to estimate their flocculation tendency. The thermal analysis and weight loss measurements were carried out using differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) respectively. For assessing the in-vitro bioactive character of synthesized glasses, the ability for apatite formation on their surface upon their immersion in simulated body fluid (SBF) was checked using SEM and pH measurements. MTS assay cytotoxicity assay and live-dead cell viability test were conducted on J774A.1 cells murine macrophage cells for different glass concentrations.

Description
Keywords
IN-VITRO BIOACTIVITY, IONIC-STRENGTH, ZINC, TEMPERATURE, CERAMICS, VIVO, NANOPARTICLES, BIOCERAMICS, SCAFFOLDS, KINETICS
Citation
Kaur, G.; Pickrell, G.; Kimsawatde, G.; Homa, D.; Allbee, H. A.; Sriranganathan, N., "Synthesis, cytotoxicity, and hydroxyapatite formation in 27-Tris-SBF for sol-gel based CaO-P2O5-SiO2-B2O3-ZnO bioactive glasses," Scientific Reports 4:4392, (2014). DOI: 10.1038/srep04392.