Baculovirus stability in serum-free lyophilized and wet storage conditions

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dc.contributor.authorColandro, Michelle Elizabethen
dc.contributor.committeechairZhang, Chenming Mikeen
dc.contributor.committeememberSenger, Ryan S.en
dc.contributor.committeememberLeRoith, Tanyaen
dc.contributor.departmentBiological Systems Engineeringen
dc.date.accessioned2015-03-05T07:00:08Zen
dc.date.available2015-03-05T07:00:08Zen
dc.date.issued2013-09-10en
dc.description.abstractThe baculovirus expression vector system (BEVS) is an effective way to produce recombinant proteins for biopharmaceuticals. However baculovirus stocks are stored in subzero temperatures to maintain virus stability, and fetal bovine serum is commonly used in the storage solution. In an effort to lower transportation and storage costs, a storage formulation that can effectively store the baculovirus in above frozen temperatures without the use of FBS would be beneficial. In this study, DMSO, ethylene glycol, glycerol, sucrose, sorbitol, sucrose-phosphate, and sucrose-phosphate-glutamate were added to baculovirus stock at various concentrations to determine the most effective stabilizer for virus storage at 4°C. Of the seven additives studied, 1 M sorbitol most effectively preserved baculovirus stock over a period of 47 weeks stored in 4°C. Formulations that include sucrose, L-arginine, and Pluronic F68 were created to determine their effectiveness on virus stability in a freeze-dried state stored at room temperature. In a lyophilized state, 0.5 M sucrose maintained baculovirus stock stability after 5 weeks of storage. Lyophilized stocks not containing sucrose were no longer infective after 5 weeks.en
dc.description.degreeMaster of Scienceen
dc.format.mediumETDen
dc.identifier.othervt_gsexam:1613en
dc.identifier.urihttp://hdl.handle.net/10919/51596en
dc.publisherVirginia Techen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectbaculovirus protein expression systemen
dc.subjectstabilityen
dc.subjectstorageen
dc.subjectlyophilizationen
dc.titleBaculovirus stability in serum-free lyophilized and wet storage conditionsen
dc.typeThesisen
thesis.degree.disciplineBiological Systems Engineeringen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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