scGFP: A Single-Cell RNA-Seq Study of Correlation Methods for Promoter-Reporter and Native Gene Expression

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dc.contributor.authorChau, Tran N.en
dc.contributor.authorAlajoleen, Razanen
dc.contributor.authorKundu, Sancharien
dc.contributor.authorBargmann, Bastiaanen
dc.contributor.authorLi, Songen
dc.date.accessioned2025-03-05T13:28:44Zen
dc.date.available2025-03-05T13:28:44Zen
dc.date.issued2025-02-21en
dc.description.abstractSingle-cell RNA sequencing (scRNA-seq) enables the exploration of transcriptomic heterogeneity at the individual cell level, facilitating the identification of distinct cell types, rare populations, and dynamic gene expression patterns over time. This technique has advanced plant biology by enabling cross-species comparisons and uncovering gene regulatory networks. While gene co-expression networks are essential for understanding these networks, commonly used correlation methods have primarily been applied to microarray and bulk RNA-seq datasets, with limited validation in single-cell contexts. This study uses an Arabidopsis scRNA-seq dataset to evaluate the performance of various correlation methods in comparing promoter-reporter genes with their native counterparts, addressing challenges such as zero-inflation and demonstrating the benefits of pseudo-bulk analysis and imputation techniques for improving correlation accuracy.en
dc.identifier.orcidLi, Song [0000-0002-8133-3944]en
dc.identifier.urihttps://hdl.handle.net/10919/124786en
dc.language.isoenen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.titlescGFP: A Single-Cell RNA-Seq Study of Correlation Methods for Promoter-Reporter and Native Gene Expressionen
dc.typePosteren
dc.type.dcmitypeTexten
pubs.organisational-groupVirginia Techen
pubs.organisational-groupVirginia Tech/Agriculture & Life Sciencesen
pubs.organisational-groupVirginia Tech/All T&R Facultyen
pubs.organisational-groupVirginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-groupVirginia Tech/Agriculture & Life Sciences/School of Plant and Environmental Sciencesen

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