Comparative analysis of the global transcriptome of Anopheles funestus from Mali, West Africa

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dc.contributor.authorSerazin, Aandrew C.en
dc.contributor.authorDana, Ali N.en
dc.contributor.authorHillenmeyer, Maureen E.en
dc.contributor.authorLobo, Neil F.en
dc.contributor.authorCoulibaly, Mamadou B.en
dc.contributor.authorWillard, Michael B.en
dc.contributor.authorHarker, Brent W.en
dc.contributor.authorSharakhov, Igor V.en
dc.contributor.authorCollins, Frank H.en
dc.contributor.authorRibeiro, Jose M. C.en
dc.contributor.authorBesansky, Nora J.en
dc.contributor.departmentEntomologyen
dc.coverage.countryUnited Statesen
dc.date.accessioned2017-01-05T22:13:56Zen
dc.date.available2017-01-05T22:13:56Zen
dc.date.issued2009-11-19en
dc.description.abstractBACKGROUND: Anopheles funestus is a principal vector of malaria across much of tropical Africa and is considered one of the most efficient of its kind, yet studies of this species have lagged behind those of its broadly sympatric congener, An. gambiae. In aid of future genomic sequencing of An. funestus, we explored the whole body transcriptome, derived from mixed stage progeny of wild-caught females from Mali, West Africa. PRINCIPAL FINDINGS: Here we report the functional annotation and comparative genomics of 2,005 expressed sequence tags (ESTs) from An. funestus, which were assembled with a previous EST set from adult female salivary glands from the same mosquito. The assembled ESTs provided for a nonredundant catalog of 1,035 transcripts excluding mitochondrial sequences. CONCLUSIONS/SIGNIFICANCE: Comparison of the An. funestus and An. gambiae transcriptomes using computational and macroarray approaches revealed a high degree of sequence identity despite an estimated 20-80 MY divergence time between lineages. A phylogenetically broader comparative genomic analysis indicated that the most rapidly evolving proteins--those involved in immunity, hematophagy, formation of extracellular structures, and hypothetical conserved proteins--are those that probably play important roles in how mosquitoes adapt to their nutritional and external environments, and therefore could be of greatest interest in disease control.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0007976en
dc.identifier.eissn1932-6203en
dc.identifier.issue11en
dc.identifier.urihttp://hdl.handle.net/10919/73960en
dc.identifier.volume4en
dc.language.isoenen
dc.relation.urihttp://www.ncbi.nlm.nih.gov/pubmed/19936243en
dc.rightsCreative Commons CC0 1.0 Universal Public Domain Dedicationen
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/en
dc.subjectAnimalsen
dc.subjectAnophelesen
dc.subjectApoptosisen
dc.subjectComputational Biologyen
dc.subjectDNA, Complementaryen
dc.subjectExpressed Sequence Tagsen
dc.subjectFemaleen
dc.subjectGene Expression Profilingen
dc.subjectGene Libraryen
dc.subjectGenomicsen
dc.subjectMalariaen
dc.subjectMalien
dc.subjectMitochondriaen
dc.subjectSalivary Glandsen
dc.subjectTranscription, Geneticen
dc.titleComparative analysis of the global transcriptome of Anopheles funestus from Mali, West Africaen
dc.title.serialPLOS ONEen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherComparative Studyen
dc.type.otherResearch Support, N.I.H., Extramuralen
dc.type.otherResearch Support, N.I.H., Intramuralen
dc.type.otherResearch Support, Non-U.S. Gov'ten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Entomologyen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen

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