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Improving the population genetics toolbox for the study of the African malaria vector Anopheles nili: microsatellite mapping to chromosomes

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dc.contributor.authorPeery, Ashleyen
dc.contributor.authorSharakhova, Maria V.en
dc.contributor.authorAntonio-Nkondjio, Christopheen
dc.contributor.authorNdo, Cyrilleen
dc.contributor.authorWeill, Myleneen
dc.contributor.authorSimard, Frédéricen
dc.contributor.authorSharakhov, Igor V.en
dc.contributor.departmentEntomologyen
dc.date.accessioned2017-01-05T22:17:39Zen
dc.date.available2017-01-05T22:17:39Zen
dc.date.issued2011-10-19en
dc.description.abstractBackground Anopheles nili is a major vector of malaria in the humid savannas and forested areas of sub-Saharan Africa. Understanding the population genetic structure and evolutionary dynamics of this species is important for the development of an adequate and targeted malaria control strategy in Africa. Chromosomal inversions and microsatellite markers are commonly used for studying the population structure of malaria mosquitoes. Physical mapping of these markers onto the chromosomes further improves the toolbox, and allows inference on the demographic and evolutionary history of the target species. Results Availability of polytene chromosomes allowed us to develop a map of microsatellite markers and to study polymorphism of chromosomal inversions. Nine microsatellite markers were mapped to unique locations on all five chromosomal arms of An. nili using fluorescent in situ hybridization (FISH). Probes were obtained from 300-483 bp-long inserts of plasmid clones and from 506-559 bp-long fragments amplified with primers designed using the An. nili genome assembly generated on an Illumina platform. Two additional loci were assigned to specific chromosome arms of An. nili based on in silico sequence similarity and chromosome synteny with Anopheles gambiae. Three microsatellites were mapped inside or in the vicinity of the polymorphic chromosomal inversions 2Rb and 2Rc. A statistically significant departure from Hardy-Weinberg equilibrium, due to a deficit in heterozygotes at the 2Rb inversion, and highly significant linkage disequilibrium between the two inversions, were detected in natural An. nili populations collected from Burkina Faso. Conclusions Our study demonstrated that next-generation sequencing can be used to improve FISH for microsatellite mapping in species with no reference genome sequence. Physical mapping of microsatellite markers in An. nili showed that their cytological locations spanned the entire five-arm complement, allowing genome-wide inferences. The knowledge about polymorphic inversions and chromosomal locations of microsatellite markers has been useful for explaining differences in genetic variability across loci and significant differentiation observed among natural populations of An. nili.en
dc.description.versionPublished versionen
dc.format.extent? - ? (10) page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.citationParasites & Vectors. 2011 Oct 19;4(1):202en
dc.identifier.doihttps://doi.org/10.1186/1756-3305-4-202en
dc.identifier.issn1756-3305en
dc.identifier.urihttp://hdl.handle.net/10919/73965en
dc.identifier.volume4en
dc.language.isoenen
dc.publisherBiomed Centralen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000297281900001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.holderAshley Peery et al.; licensee BioMed Central Ltd.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectParasitologyen
dc.subjectPARASITOLOGYen
dc.subjectChromosome inversionsen
dc.subjectgenome sequenceen
dc.subjectmalaria vectoren
dc.subjectmicrosatellite markersen
dc.subjectpopulation structureen
dc.subjectMICROARRAY-BASED ANALYSISen
dc.subjectPLASMODIUM-FALCIPARUMen
dc.subjectTEMPORAL STABILITYen
dc.subjectCULICIDAE LARVAEen
dc.subjectGAMBIAE COMPLEXen
dc.subjectSARCOPTES MITEen
dc.subjectWEST-AFRICAen
dc.subjectCAMEROONen
dc.subjectTRANSMISSIONen
dc.subjectFUNESTUSen
dc.titleImproving the population genetics toolbox for the study of the African malaria vector Anopheles nili: microsatellite mapping to chromosomesen
dc.title.serialParasites & Vectorsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Entomologyen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen

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