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Strength Lies in Diversity: How Community Diversity Limits Salmonella Abundance in the Chicken Intestine

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dc.contributor.authorPedroso, Adriana A.en
dc.contributor.authorLee, Margie D.en
dc.contributor.authorMaurer, John J.en
dc.date.accessioned2021-12-14T15:09:57Zen
dc.date.available2021-12-14T15:09:57Zen
dc.date.issued2021-06-15en
dc.date.updated2021-12-14T15:09:53Zen
dc.description.abstractThe transfer of the intestinal microbiota from adult to juvenile animals reduces Salmonella prevalence and abundance. The mechanism behind this exclusion is unknown, however, certain member species may exclude or promote pathogen colonization and Salmonella abundance in chickens correlates with intestinal community composition. In this study, newly hatched chicks were colonized with Salmonella Typhimurium and 16S rRNA libraries were generated from the cecal bacterial community at 21, 28, 35, and 42 days of age. Salmonella was quantified by real-time PCR. Operational taxonomic units (OTUs) were assigned, and taxonomic assignments were made, using the Ribosomal Database Project. Bacterial diversity was inversely proportional to the Salmonella abundance in the chicken cecum (p < 0.01). In addition, cecal communities with no detectable Salmonella (exclusive community) displayed an increase in the abundance of OTUs related to specific clostridial families (Ruminococcaceae, Eubacteriaceae, and Oscillospiraceae), genera (Faecalibacterium and Turicibacter) and member species (Ethanoligenens harbinense, Oscillibacter ruminantium, and Faecalibacterium prausnitzii). For cecal communities with high Salmonella abundance (permissive community), there was a positive correlation with the presence of unclassified Lachnospiraceae, clostridial genera Blautia and clostridial species Roseburia hominis, Eubacterium biforme, and Robinsoniella peoriensis. These findings strongly support the link between the intestinal bacterial species diversity and the presence of specific member species with Salmonella abundance in the chicken ceca. Exclusive bacterial species could prove effective as direct-fed microbials for reducing Salmonella in poultry while permissive species could be used to predict which birds will be super-shedders.en
dc.description.versionPublished versionen
dc.format.extent12 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifierARTN 694215 (Article number)en
dc.identifier.doihttps://doi.org/10.3389/fmicb.2021.694215en
dc.identifier.eissn1664-302Xen
dc.identifier.issn1664-302Xen
dc.identifier.orcidMaurer, John [0000-0001-8680-7005]en
dc.identifier.pmid34211451en
dc.identifier.urihttp://hdl.handle.net/10919/106977en
dc.identifier.volume12en
dc.language.isoenen
dc.publisherFrontiersen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000667666200001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectLife Sciences & Biomedicineen
dc.subjectMicrobiologyen
dc.subjectSalmonellaen
dc.subjectchickenen
dc.subjectbroileren
dc.subjectintestineen
dc.subjectdiversityen
dc.subjectClostridiaen
dc.subjectREAL-TIME PCRen
dc.subjectCLOSTRIDIUM-DIFFICILE INFECTIONen
dc.subjectGUT MICROBIOTAen
dc.subjectPHYLOGENETIC ANALYSISen
dc.subjectTYPHIMURIUMen
dc.subjectCOLONIZATIONen
dc.subjectINFLAMMATIONen
dc.subjectESTABLISHMENTen
dc.subjectENTERITISen
dc.subjectDEPLETIONen
dc.subject0502 Environmental Science and Managementen
dc.subject0503 Soil Sciencesen
dc.subject0605 Microbiologyen
dc.titleStrength Lies in Diversity: How Community Diversity Limits Salmonella Abundance in the Chicken Intestineen
dc.title.serialFrontiers in Microbiologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
dcterms.dateAccepted2021-05-24en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Animal and Poultry Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen

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