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Photonic Biosensor Assays to Detect and Distinguish Subspecies of Francisella tularensis

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dc.contributor.authorCooper, Kristie L.en
dc.contributor.authorBandara, Aloka B.en
dc.contributor.authorWang, Yunmiaoen
dc.contributor.authorWang, Anboen
dc.contributor.authorInzana, Thomas J.en
dc.contributor.departmentElectrical and Computer Engineeringen
dc.contributor.departmentCenter for Photonics Technologyen
dc.contributor.departmentBiomedical Sciences and Pathobiologyen
dc.contributor.departmentHuman Nutrition, Foods, and Exerciseen
dc.contributor.departmentFralin Life Sciences Instituteen
dc.date.accessioned2018-01-16T14:47:21Zen
dc.date.available2018-01-16T14:47:21Zen
dc.date.issued2011-03-07en
dc.description.abstractThe application of photonic biosensor assays to diagnose the category-A select agent Francisella tularensis was investigated. Both interferometric and long period fiber grating sensing structures were successfully demonstrated; both these sensors are capable of detecting the optical changes induced by either immunological binding or DNA hybridization. Detection was made possible by the attachment of DNA probes or immunoglobulins (IgG) directly to the fiber surface via layer-by-layer electrostatic self-assembly. An optical fiber biosensor was tested using a standard transmission mode long period fiber grating of length 15 mm and period 260 µm, and coated with the IgG fraction of antiserum to F. tularensis. The IgG was deposited onto the optical fiber surface in a nanostructured film, and the resulting refractive index change was measured using spectroscopic ellipsometry. The presence of F. tularensis was detected from the decrease of peak wavelength caused by binding of specific antigen. Detection and differentiation of F. tularensis subspecies tularensis (type A strain TI0902) and subspecies holarctica (type B strain LVS) was further accomplished using a single-mode multi-cavity fiber Fabry-Perot interferometric sensor. These sensors were prepared by depositing seven polymer bilayers onto the fiber tip followed by attaching one of two DNA probes: (a) a 101-bp probe from the yhhW gene unique to type-A strains, or (b) a 117-bp probe of the lpnA gene, common to both type-A and type-B strains. The yhhW probe was reactive with the type-A, but not the type-B strain. Probe lpnA was reactive with both type-A and type-B strains. Nanogram quantities of the target DNA could be detected, highlighting the sensitivity of this method for DNA detection without the use of PCR. The DNA probe reacted with 100% homologous target DNA, but did not react with sequences containing 2-bp mismatches, indicating the high specificity of the assay. These assays will fill an important void that exists for rapid, culture-free, and field-compatible diagnosis of F. tularensis.en
dc.description.versionPublished versionen
dc.format.extent3004 - 3019 (16) page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.citationCooper, K.L.; Bandara, A.B.; Wang, Y.; Wang, A.; Inzana, T.J. Photonic Biosensor Assays to Detect and Distinguish Subspecies of Francisella tularensis. Sensors 2011, 11, 3004-3019.en
dc.identifier.doihttps://doi.org/10.3390/s110303004en
dc.identifier.issn1424-8220en
dc.identifier.issue3en
dc.identifier.urihttp://hdl.handle.net/10919/81799en
dc.identifier.volume11en
dc.language.isoenen
dc.publisherMDPIen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000288786900044&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectTechnologyen
dc.subjectChemistry, Analyticalen
dc.subjectElectrochemistryen
dc.subjectInstruments & Instrumentationen
dc.subjectChemistryen
dc.subjectFrancisella tularensisen
dc.subjectdiagnosisen
dc.subjectlong period fiber gratingen
dc.subjectfiber Fabry-Perot interferometric sensoren
dc.subjectprobeen
dc.subjectantibodyen
dc.subjectDNAen
dc.subjectDNA HYBRIDIZATIONen
dc.subjectREFRACTIVE-INDEXen
dc.subjectACTINOBACILLUS-PLEUROPNEUMONIAEen
dc.subjectSEROTYPE SPECIFICITYen
dc.subjectCAPSULAR POLYMERen
dc.subjectSENSORen
dc.subjectFILMSen
dc.subjectTULAREMIAen
dc.subjectDIAGNOSISen
dc.subjectPRESSUREen
dc.titlePhotonic Biosensor Assays to Detect and Distinguish Subspecies of Francisella tularensisen
dc.title.serialSensorsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Human Nutrition, Foods, & Exerciseen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Engineeringen
pubs.organisational-group/Virginia Tech/Engineering/COE T&R Facultyen
pubs.organisational-group/Virginia Tech/Engineering/Electrical and Computer Engineeringen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/University Research Institutesen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciencesen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences/Fralin Affiliated Facultyen
pubs.organisational-group/Virginia Tech/Veterinary Medicineen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/Biomedical Sciences and Pathobiologyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/CVM T&R Facultyen

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