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The Application of a Nanomaterial Optical Fiber Biosensor Assay for Identification of Brucella Nomenspecies

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dc.contributor.authorMcCutcheon, Kellyen
dc.contributor.authorBandara, Aloka B.en
dc.contributor.authorZuo, Ziweien
dc.contributor.authorHeflin, James R.en
dc.contributor.authorInzana, Thomas J.en
dc.contributor.departmentBiomedical Sciences and Pathobiologyen
dc.contributor.departmentPhysicsen
dc.date.accessioned2019-05-31T11:52:05Zen
dc.date.available2019-05-31T11:52:05Zen
dc.date.issued2019-05-21en
dc.date.updated2019-05-31T06:01:59Zen
dc.description.abstractBacteria in the genus <i>Brucella</i> are the cause of brucellosis in humans and many domestic and wild animals. A rapid and culture-free detection assay to detect <i>Brucella</i> in clinical samples would be highly valuable. Nanomaterial optical fiber biosensors (NOFS) are capable of recognizing DNA hybridization events or other analyte interactions with high specificity and sensitivity. Therefore, a NOFS assay was developed to detect <i>Brucella</i> DNA from cultures and in tissue samples from infected mice. An ionic self-assembled multilayer (ISAM) film was coupled to a long-period grating optical fiber, and a nucleotide probe complementary to the <i>Brucella</i> IS<i>711</i> region and modified with biotin was bound to the ISAM by covalent conjugation. When the ISAM/probe duplex was exposed to lysate containing &ge;100 killed cells of <i>Brucella</i>, or liver or spleen tissue extracts from <i>Brucella-</i>infected mice, substantial attenuation of light transmission occurred, whereas exposure of the complexed fiber to non-<i>Brucella</i> gram-negative bacteria or control tissue samples resulted in negligible attenuation of light transmission. Oligonucleotide probes specific for <i>B. abortus</i>, <i>B. melitensis</i>, and <i>B. suis</i> could also be used to detect and differentiate these three nomenspecies. In summary, the NOFS biosensor assay detected three nomenspecies of <i>Brucella</i> without the use of polymerase chain reaction within 30 min and could specifically detect low numbers of this bacterium in clinical samples.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationMcCutcheon, K.; Bandara, A.B.; Zuo, Z.; Heflin, J.R.; Inzana, T.J. The Application of a Nanomaterial Optical Fiber Biosensor Assay for Identification of Brucella Nomenspecies. Biosensors 2019, 9, 64.en
dc.identifier.doihttps://doi.org/10.3390/bios9020064en
dc.identifier.urihttp://hdl.handle.net/10919/89653en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectBrucella abortusen
dc.subjectBrucella melitensisen
dc.subjectBrucella suisen
dc.subjectoptical fiberen
dc.subjectbiosensoren
dc.subjectnucleotide probeen
dc.subjectlight transmissionen
dc.subjectdiagnosisen
dc.titleThe Application of a Nanomaterial Optical Fiber Biosensor Assay for Identification of Brucella Nomenspeciesen
dc.title.serialBiosensorsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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