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dc.contributor.authorZellner, Phillipen_US
dc.contributor.authorShake, Tyleren_US
dc.contributor.authorSahari, Alien_US
dc.contributor.authorBehkam, Baharehen_US
dc.contributor.authorAgah, Masouden_US
dc.date.accessioned2017-02-12T22:37:42Z
dc.date.available2017-02-12T22:37:42Z
dc.date.issued2013-08-01en_US
dc.identifier.issn1618-2642en_US
dc.identifier.urihttp://hdl.handle.net/10919/75001
dc.description.abstractIn this study, we report the first off-chip passivated-electrode, insulator-based dielectrophoresis microchip (OπDEP). This technique combines the sensitivity of electrode-based dielectrophoresis (eDEP) with the high throughput and inexpensive device characteristics of insulator-based dielectrophoresis (iDEP). The device is composed of a permanent, reusable set of electrodes and a disposable, polymer microfluidic chip with microposts embedded in the microchannel. The device operates by capacitively coupling the electric fields into the microchannel; thus, no physical connections are made between the electrodes and the microfluidic device. During operation, the polydimethylsiloxan (PDMS) microfluidic chip fits onto the electrode substrate as a disposable cartridge. OπDEP uses insulting structures within the channel as well as parallel electrodes to create DEP forces by the same working principle that iDEP devices use. The resulting devices create DEP forces which are larger by two orders of magnitude for the same applied voltage when compared to off-chip eDEP designs from literature, which rely on parallel electrodes alone to produce the DEP forces. The larger DEP forces allow the OπDEP device to operate at high flow rates exceeding 1 mL/h. In order to demonstrate this technology, Escherichia coli (E. coli), a known waterborne pathogen, was trapped from water samples. Trapping efficiencies of 100 % were obtained at flow rates as high as 400 μL/h and 60 % at flow rates as high as 1200 μL/h. Additionally, bacteria were selectively concentrated from a suspension of polystyrene beads.
dc.format.extent6657 - 6666 (10) page(s)en_US
dc.languageEnglishen_US
dc.publisherSpringer Heidelbergen_US
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000322705600006&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en_US
dc.subjectBiochemical Research Methodsen_US
dc.subjectAnalytical Chemistryen_US
dc.subjectBiochemistryen_US
dc.subjectMolecular Biologyen_US
dc.subjectDielectrophoresis (DEP)en_US
dc.subjectMicrofluidicen_US
dc.subjectInsulator-based dielectrophoresis (iDEP)en_US
dc.subjectEscherichia coli (E. coli)en_US
dc.subjectMicrofabricationen_US
dc.titleOff-chip passivated-electrode, insulator-based dielectrophoresis (O pi DEP)en_US
dc.typeArticle - Refereed
dc.description.versionPublisheden_US
dc.title.serialANALYTICAL AND BIOANALYTICAL CHEMISTRYen_US
dc.identifier.doihttps://doi.org/10.1007/s00216-013-7123-7
dc.identifier.volume405en_US
dc.identifier.issue21en_US
pubs.organisational-group/Virginia Tech
pubs.organisational-group/Virginia Tech/All T&R Faculty
pubs.organisational-group/Virginia Tech/Engineering
pubs.organisational-group/Virginia Tech/Engineering/COE T&R Faculty
pubs.organisational-group/Virginia Tech/Engineering/Electrical and Computer Engineering
pubs.organisational-group/Virginia Tech/Engineering/Mechanical Engineering
pubs.organisational-group/Virginia Tech/Faculty of Health Sciences


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