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High protective efficacy of rice bran against human rotavirus diarrhea via enhancing probiotic growth, gut barrier function, and innate immunity

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dc.contributor.authorYang, Xingdongen
dc.contributor.authorTwitchell, Ericaen
dc.contributor.authorLi, Guohuaen
dc.contributor.authorWen, Keen
dc.contributor.authorWeiss, Mariahen
dc.contributor.authorKocher, Jacoben
dc.contributor.authorLei, Shaohuaen
dc.contributor.authorRamesh, Ashwinen
dc.contributor.authorRyan, Elizabeth P.en
dc.contributor.authorYuan, Lijuanen
dc.date.accessioned2017-02-22T15:36:41Zen
dc.date.available2017-02-22T15:36:41Zen
dc.date.issued2015-10-13en
dc.description.abstractPreviously, we showed that rice bran (RB) was able to reduce human rotavirus (HRV) diarrhea in gnotobiotic pigs. Here, we investigated its effect on the growth of diarrhea-reducing probiotic Lactobacillus rhamnosus GG (LGG) and Escherichia coli Nissle (EcN), and the resulting effects on HRV diarrhea, gut epithelial health, permeability and innate immune responses during virulent HRV challenge. On 3, 5, and 7 days of age pigs were inoculated with 2 × 104 colony-formingunits LGG+EcN to initiate colonization. Daily RB supplementation (replacing 10% calorie intake) was started at 5 days of age and continued until euthanasia. A subset of pigs in each group was challenged orally with 105 focus-forming-units of virulent HRV at 33 days of age. RB completely prevented HRV diarrhea in LGG+EcN colonized pigs. RB significantly promoted the growth of both probiotic strains in the gut (~5 logs) and increased the body-weight-gain at 4–5 weeks of age compared to non-RB group. After HRV challenge, RB-fed pigs had significantly lower ileal mitotic index and villus width, and significantly increased intestinal IFN-γ and total IgA levels compared to non-RB group. Therefore, RB plus LGG+EcN colonization may represent a highly effective therapeutic approach against HRV and potentially a variety of other diarrhea-inducing enteric pathogens.en
dc.description.versionPublished versionen
dc.format.extent12 pagesen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1038/srep15004en
dc.identifier.issn2045-2322en
dc.identifier.urihttp://hdl.handle.net/10919/75124en
dc.identifier.volume5en
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000362634400001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectlactobacillus-rhamnosus ggen
dc.subjectgnotobiotic pig modelen
dc.subjectrandomized controlled-trialen
dc.subjectantibody-responsesen
dc.subjectvaccineen
dc.subjectchildrenen
dc.subjectpigletsen
dc.subjectmucosalen
dc.subjectcellsen
dc.subjectratsen
dc.titleHigh protective efficacy of rice bran against human rotavirus diarrhea via enhancing probiotic growth, gut barrier function, and innate immunityen
dc.title.serialScientific Reportsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/Veterinary Medicineen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/Biomedical Sciences and Pathobiologyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/CVM T&R Facultyen

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